https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Mechanisms and effects posed by neurotoxic products of cyanobacteria/microbial eukaryotes/dinoflagellates in algae blooms: a review https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36465 Wed 13 May 2020 11:32:41 AEST ]]> Cyanobacterial community composition and bacteria-bacteria interactions promote the stable occurrence of particle-associated bacteria https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32385 Mon 23 Sep 2019 13:27:12 AEST ]]> Mutagenesis of the microcystin tailoring and transport proteins in a heterologous cyanotoxin expression system https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48515 mcyA-J. The recent establishment of a cyanotoxin heterologous expression system in Escherichia coli has provided the means to study microcystin biosynthesis in a genetically tractable, rapidly growing host. Using this system, we demonstrate that deletion of the ABC-transporter, mcyH, and dehydrogenase, mcyI, abolishes microcystin production, while deletion of the O-methyltransferase, mcyJ, results in the production of the demethylated (DM) toxin [D-Asp³ , DMAdda ⁵]microcystin-LR. Both methylated and DM toxin variants were heterologously produced at high titers and efficiently exported into the extracellular medium, enabling easy purification. The results show that the mcy gene cluster can be engineered in E. coli to study the function of its individual components and direct the synthesis of particular microcystin variants. This technology could potentially be applied to other natural products of ecological and biomedical significance.]]> Mon 20 Mar 2023 16:46:36 AEDT ]]> An In Vitro and In Vivo Study of Broad-Range Phosphopantetheinyl Transferases for Heterologous Expression of Cyanobacterial Natural Products https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43382 Fri 16 Sep 2022 09:23:25 AEST ]]>